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Some PCR Tests Key on Naturally-Occurring Human DNA to Render False Positive COVID-19 Result


None of the PCR Tests currently in use were validated or confirmed to be true and accurate measurements for COVID-19 Detection or Diagnosis



 Above:  The primer sequence for one of many COVID-19 PCR test protocols matches the DNA sequence for human chromosome 8 exactly.  This may cause anyone tested with this test to appear “positive” for COVID-19 because the test is designed to identify the naturally occurring human chromosome.             graphic/David Deschesne



By:  David Deschesne

September 9, 2020


   COVID-19 PCR test protocols have been hastily built using dirty samples with non-isolated and non-purified virus particles as the US FDA suspended all standards and verification of testing accuracy under the COVID-19 “emergency” last March.

   At least one test protocol being used by some manufacturers of the COVID-19 test has been revealed to key in on a DNA strand found naturally in a human chromosome - which everybody has - thus causing a “positive” result for COVID-19 when there may be no real infection, at all.  

   The gene primer sequence for COVID-19 is shown on the World Health Organization’s website for COVID-19 PCR testing protocols1.  Curiously, the same sequence is also found on the National Institute of Health’s website as a sequence in normal human DNA2.   The research originally appeared in an article on 3.  The author of the article stated, “The primer sequences are what get amplified by the PCR process in order to be detected and designated a 'positive' test result.   This is the WHO protocol.  I also came across several private companies (Sigma-Aldrich, Thermo Fisher) whose PCR sequences were longer and had no such match.  So, I am not saying that ALL test kits belong to WHO, but if we were to find shenanigans we would expect to find it with the WHO.” 

   The author, who goes by the pseudonym, Fauxlex also pointed out the fact that the PCR test process is inherently error-prone and even the WHO test may occasionally have false negatives. “You would think they would have some technology to pick PCR primers that selects against sequences already found within human DNA.  They also should have gone with a primer longer than 18 characters if they want reliable results.  These tests are going to have a huge rate of false positives and/or negatives to the point where it’s questionable how much we can rely upon it.  Let alone the idea that 80% of people who test positive show no outward symptoms.  We need to end these draconian lockdowns immediately.”

   The author was then challenged in the comments section of his story by a commenter named Bernd Payson who pointed out that the PCR is looking for RNA, and the chromosome 8 character string is for DNA so “it’s not going to be replicated.”  The author responded, “I disagree that the process could not possibly be thrown off by error.  Any instances where chromosome 8 had been unzipped for replication would leave the matching sequence available for the PCR primer to anneal.  This might not cause full replication by the PCR process, but if the test is so touchy you would really think this could throw off their results.”

   Dr. Andrew Kaufman was recently interviewed by David Icke from to go over this new revelation in particular and the PCR process in general.4  Dr. Kaufman is a  Doctor of Medicine and former medical instructor in hematology and oncology at the University of South Carolina.  He also has a B.S. in Biology at M.I.T.; is an assistant professor of Psychiatry and is licensed and board certified in Psychiatry and Forensic Psychiatry.  Additionally, Dr. Kaufman also has experience in computer modeling in the medical field.


Mechanics of a PCR Test

   The way a PCR test works is a swab is taken from mucous membranes inside the nose or throat which contain many different cells and different organisms.  “It will contain a mixture of human cells, virus particles and other microbes which could mean bacteria, or fungi or other microbes,” explained Dr. Kaufman.  “So, it's not a clean sample and if we were testing for human DNA or RNA because there are human cells in there and also fragments of human cells, such as apoptotic bodies, or exosomes or sometimes there's even free human DNA circulating in some of our fluids.”

   Since there's not enough viral RNA to detect directly in the patient's sample, a process called Reverse Transcription Polymerase Chain Reaction (RT-PCR) amplifies many copies of a segment of the N gene to detectable levels.  “There's a tiny, tiny amount of the RNA that we're looking for in this sample that's really messy and contains a lot of stuff so in order to be able to detect it, at such tiny, tiny amounts they have to amplify it or make multiple copies.  That's really what the PCR test does,” said Dr. Kaufman.

    Short, single stranded pieces of DNA called primers are designed to recognize unique RNA sequences within the viral genome that bracket the target region of the N gene. After the first primer binds, an enzyme called reverse transcriptase extends (synthesizes) a single-stranded DNA copy of the viral RNA, known as complementary DNA, or cDNA.

   “This really shows the important part that we need to pay attention to,” said Dr. Kaufman.  “There's a short primer.  That's the only thing that we have identified in advance.  We created the primer, we can make a primer for any sequence for any organism.  Then when we put that in this reaction. Basically we're looking for a piece of genetic material from some organism that matches it - that is the 'compliment.'  When these two strands go together, they have a complimentarity, it's like a plus and a minus that attract each other.  It's G's, C's, A's ant T's.  So, this is going to look for a specific sequence in the sample that we're trying to find.  Since the primer we're talking about is an exact sequence for chromosome 8, then it could identify a piece of a transcript from chromosome 8 rather than some genetic material from another organism.  In other words, it can detect our own DNA.”

   When asked about the research connecting the WHO PCR test protocol to a DNA fragment found in the human genome, Dr. Kaufman was certainly intrigued since he had been conducting similar research earlier this year.  “I knew this was a very significant thing but I wanted to take my time and verify it and learn a little bit more about it before speaking about it.  I tried to do this kind of research back in April because I suspected there would be serious overlap between some of the target sequences they're looking for with the PCR test and our human genome because of this confusion about exosomes and what RNA comes from what source in all of this.  I looked at a different protocol than the one published here.  I looked at one from Germany  - this one is from the Pasteur Institute in France, but it really confirmed my suspicion and I was glad to see it. Basically one of the primer sequences in the PCR tests, according to the Pasteur Institute protocol is an exact match for a sequence in our own human DNA on chromosome 8.”

   This is one of the primary reasons the creator of the RT-PCR process, Kerry Mullis, said it should never be used for diagnostic purposes, but that is exactly what politicians and bureaucrats the world over are doing with COVID-19.


PCR Primer -

Human Genome Connection

   After explaining the PCR process and the implications of this current research, David Icke then attempted to clarify by stating, “So, correct me if I'm wrong, where the PCR test here is testing for potentially material that's in the body as a matter of course and if the PCR test gives a positive they're calling it the virus SARS-CoV-2.  Is that correct?”

   “Yes, but it's a little more complicated than that because there's more than one primer that they can use,” explained Dr. Kaufman.  “This whole testing thing is very, very heterogeneous around the world. In other words, there are different protocols in virtually every country.  A lot of them are recommended through the World Health Organization but there are some from other bodies, too.  They can use different primers.  Even when they do just one sample, they're not going to use just one primer for a test and say if that one's positive it's positive, so it's not exactly that clear cut.  But what we're talking about here is at least one of the primers that they're using in one of the tests tests for our own [human] sequence.  So, this could come up positive in any human regardless of the presence of any virus or anything else.  It adds another level of confusion and obfuscation to how do you interpret the results of a test like this.” 

   Dr. Kaufman then further reiterated his earlier point about dirty samples.  “I still think that the most salient point is that we don't know where these sequences came from because there was never a purified particle of a virus where they had a bunch of them together in one sample and they could characterize those and pull the RNA material right out of those particles.  They never did it that way.  They only looked at it in this dirty sample, which was a mixture of many, many microorganisms and human cells.  They pulled these sequences out of there because they thought the sequences belonged to the family  of coronaviruses.  But, that was based on only about 80 percent sequence identity between those samples.  When I looked and did that research previously, with the other probes for a different protocol I found that many of those primers in that sequence had a eighty to eighty-five percent sequence identity with stretches in the human genome, as well. I just didn't find a complete match.”

     Kaufman also expanded on the difficulty in using a PCR test for diagnostic purposes.  “There's a lot of overlap in this and if you look at the nature of the PCR test itself, the enzymes that they use to copy the RNA make mistakes.  So, they could give you a lot of false positives.  This is one of the problems that's always been known about this test the more you amplify it.  Because you do this test in cycles, in each cycle you double the copies of that piece of RNA that you're looking for.  The more times you double it, the more false signals that you get because you're also amplifying the noise signal which could be other sequences that partially matched and they're not detectable until you amplify them so many times and then it's like everything that is an artifact in there is also detectable and that gives you a false positive.  That's one of the main reasons why Kerry Mullis did not recommend using this as a diagnostic test, because of all those false positives.”

  “In my opinion, since we don't know the origin of the sequences that we're testing - other than now we know one of them may come from our own sequences, like the human sequence - then there's really no way to calculate any accuracy,” said Dr. Kaufman.  “I would say there's a one hundred percent error rate with [the PCR] test.”